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. 2019 Aug 2;10:1839. doi: 10.3389/fimmu.2019.01839

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Copyright © 2019 Chiang, Wu, Chen, Liu, Leng and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD8⁺ dendritic cells can mediate rOVA-FLIPr induction of T cell activation in vivo. C57BL/6 mice were injected with rOVA-FLIPr in the hind foot pads (50 μg/foot pad). Lymph nodes were harvested 72 h after injection. Graded numbers of sorted CD8⁺ DCs and CD8⁻ DCs were cocultured with OT-2 and OT-1 cells for 24 and 40 h, respectively. Levels of IL-2 and IFN-γ in the supernatants were determined by ELISA. Representative results (mean ± SEM) of 2 separate experiments performed in duplicate wells.