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. 2019 Aug 2;10:520. doi: 10.3389/fendo.2019.00520

Figure 4.

Figure 4

Growing follicles, defined as percentages of primary, secondary, and antral follicles out of the total number of follicles, in fresh, slow-frozen-thawed, and vitrified-warmed ovarian tissues. The percentage of primordial, intermediate, primary, secondary, and antral follicles was calculated out of total follicles for each group (fresh, slow-frozen, vitrified) and subgroup (fresh, after thawing/warming, no culture, 48 h culture, 48 h IVA). Intermediate, primary, secondary, and antral follicles were considered as growing follicles and the pool of inactivated primordial follicles was compared between the three different groups. Percentages of primordial follicles in the 48 h IVA and 48 h culture subgroups were 24.49 vs. 32.32%, p = 0.78, 17.65% vs. 14.29%, p = 0.99, and 10.53 vs. 8.33%, p = 0.92, for fresh, slow-frozen, and vitrified tissues, respectively.