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. 2019 May 20;28(16):2775–2784. doi: 10.1093/hmg/ddz106

Figure 3.

Figure 3

Dnaaf2 −/− embryos have a morphologically distinct node. Embryos were collected at embryonic day (E) 8.5 and examined under a microscope for node assessment (A and B). Dnaaf2−/− embryos do have a morphologically distinct node (black hollow triangle in A′ and B′). (A′ and B′) Magnified view of node region is indicated with a black arrow. The construct of the Dnaaf2 KO allele is generated by replacing a portion of the Dnaaf2 genomic sequence with a cassette containing a LacZ reporter gene (C). ISH F1 and ISH R1 denote the intron-spanning primers used for the generation of Dnaaf2 ISH probe. X-gal staining reveals LacZ expression precisely in the node but not in any other tissues (black triangle in D). ISH F1 and ISH R1 are the forward and reverse primers for Dnaaf2 ISH probe generation. Frontal view, A and B; lateral view, D and E; sagittal section, E. +/− denotes Dnaaf2 heterozygous embryo; −/− denotes Dnaaf2 mutant embryo.