Figure 3.
IL‐2 influences the BCL‐2 Rheostat. A, mRNA for BCL‐2 was elevated and BIM was reduced when Th2 cells were cultured in high IL‐2 (5 ng/mL) compared to low IL‐2 (1.25 ng/mL). B, Genome browser tracks for STAT5 ChIP under control and IL‐2 replete conditions within the BCL‐2 gene body. C, High IL‐2 increases the BCL‐2:BIM ratio (n = 7). D, The BCL‐2:BIM ratio remains strong (above 1) even after DEX treatment (24 hours, n = 3). E, Th2 cells cultured in high IL‐2 and DEX had greater BCL‐2:BIM ratios than low IL‐2 and DEX (10−8–10−6M combined, n = 9). Data are presented as mean ± standard error. *P < 0.05 vs no DEX (for each IL‐2 concentration); # P < 0.05, high vs low IL‐2. ChIP, chromatin immunoprecipitation; DEX, dexamethasone; IL‐2, interleukin 2; mRNA, messenger RNA; STAT5, signal transducer and activator of transcription 5; Th2, T‐helper cell