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. 2019 Apr 17;7(3):112–124. doi: 10.1002/iid3.249

Figure 5.

Figure 5

GR abundance and function are decreased by IL‐2. A, Th2 cells cultured with high IL‐2 (5 ng/mL) had significantly less GR mRNA compared to those cultured in low IL‐2 (1.25 ng/mL; n = 6). B, GRβ mRNA was low in Th2 cells and was unaffected by IL‐2 levels. C, FKBP5, a known GC inducible gene, had significantly reduced expression under high IL‐2 high. D, Chromatin immunoprecipitation (ChIP) for binding of GR to a regulatory element within the FKBP5 locus. GR binding was significantly less in Th2 cells cultured in high IL‐2 (5 ng/mL) compared to low IL‐2 (1.25 ng/mL). E, Expression of GR and IL‐13 mRNA were inversely correlated. Data are presented as mean ± standard error. *P < 0.05 comparison of DEX vs vehicle for each IL‐2 concentration; # P < 0.05, comparison of each DEX concentration high vs low IL‐2. DEX, dexamethasone; GR, glucocorticoid receptor; IL‐2, interleukin 2; mRNA, messenger RNA; Th2, T‐helper cell