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. 2019 Aug 2;9:279. doi: 10.3389/fcimb.2019.00279

Figure 4.

Figure 4

Virulence and infection progression of S. Typhimurium mutants in Rubicon-depleted hosts. (A) Work flow and time line of experiments followed in (B–K). (B,D,F,H,J) Survival curves for systemically injected S. Typhimurium mutant strains, ΔphoP (B), ΔpurA (D), ΔsipB (F), ΔssrB (H), and ΔflhD (J) in Rubicon-depleted larvae with their respective control groups. One representative of two replicates is shown (n = 50 embryos per group). (C,E,G,I,K) Representative CFU counts of the infections with S. Typhimurium mutant strains, ΔphoP (C), ΔpurA (E), ΔsipB (G), ΔssrB (I), and ΔflhD (K) in Rubicon-depleted zebrafish with their respective control groups at 24 hpi. One representative of two replicates is shown, where five embryos per time point were used and the log transformed CFU data are shown with the geometric mean per time point. Error bars represent SD. ****P < 0.0001, **P < 0.01, *P < 0.05, ns, non-significant.