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. 2019 Aug 5;50(3):296–312.e5. doi: 10.1016/j.devcel.2019.05.020
Reagent or Resource Source Identifier
Antibodies
Rabbit polyclonal anti-GFP Abcam Cat# ab6556; RRID: AB_305564
Goat polyclonal anti-GFP Abcam Cat# ab6673; RRID: AB_305643
Rabbit polyclonal anti-mCherry Abcam Cat# ab183628; RRID: AB_2650480
Mouse monoclonal anti-RFP Abcam Cat# ab65856; RRID: AB_1141717
Rat monoclonal anti-αtubulin [YL1/2] Abcam Cat# ab6160; RRID: AB_305328
Mouse monoclonal anti-acetylated αtubulin Abcam Cat# ab24610; RRID: AB_448182
Rabbit monoclonal anti-Smad3 (phospho S423 + S425) [EP823Y] Abcam Cat# ab52903; RRID: AB_882596
Mouse monoclonal anti-γtubulin, Clone GTU-88 Sigma Cat# NB 100-1628; RRID: AB_523854
Rabbit polyclonal anti-Vasa (d-260) Santa Cruz Cat# sc-30210; RRID: AB_793874
Mouse monoclonal anti-αSpectrin DSHB Cat# 3A9 (323 or M10-2); RRID: AB_528473
Rat monoclonal anti-E-cadherin DSHB Cat# DCAD2; RRID: AB_528120
Mouse monoclonal anti-futsch DSHB Cat# 22c10; RRID: AB_528403
Mouse Anti-HA Monoclonal Clone 12CA5 Roche Cat# 11666606001; RRID: AB_514506
Donkey anti-Rabbit IgG Secondary Antibody, Alexa Fluor 488 Thermofisher Cat# R37118; RRID: AB_2556546
Donkey anti-Goat IgG Secondary Antibody, Alexa Fluor 488 Thermofisher Cat# A-11055; RRID: AB_2534102
Donkey anti-Mouse IgG Secondary Antibody, Alexa Fluor 555 Thermofisher Cat# A-31570; RRID: AB_2536180
Donkey anti-Rabbit IgG Secondary Antibody, Alexa Fluor 555 Thermofisher Cat# A-31572; RRID: AB_162543
Donkey anti-Rat IgG Secondary Antibody, Alexa Fluor 594 Thermofisher Cat# A-21209; RRID: AB_2535795
Donkey anti-Rabbit IgG Secondary Antibody, Alexa Fluor 647 Thermofisher Cat# A-31573; RRID: AB_2536183
Chicken anti-Rat IgG Secondary Antibody, Alexa Fluor 647 Thermofisher Cat# A-21472; RRID: AB_2535875
Chemicals, Peptides, and Recombinant Proteins
Collagenase type IV Worthington Biochemicals LS004186
TRIzol Invitrogen 15596-018
Texas Red-X Phalloidin Invitrogen T7471
Prolong Gold Antifade with DAPI Invitrogen P36935
Dextran, Texas Red, 3000 MW, Neutral Invitrogen D3329
Fetal bovine serum Sigma F3018
Penicillin-Streptomycin Sigma P0781
Human insulin Sigma I2643
Paclitaxel Sigma T7402
Nocodazole Sigma SML1665
Cytochalasin D Sigma C2618
Bafilomycin A1 Sigma B1793
Dynasore Sigma D7693
Schneider's Drosophila Medium Thermofisher 21720-024
Fibrinogen, Bovine Plasma Millipore 341573
Thrombin protease GE Healthcare Lifesciences 27-0846-01
Experimental Models: Organisms/Strains
D. melanogaster; w∗;
P{w+mC=vas.EGFP.HA}2 		DDGR (Kyoto) Cat# 109171; RRID:DGGR_109171
D. melanogaster; w∗;
bam-GFP 		Chen and McKearin, 2003 N/A
D. melanogaster; w∗;
UASp-tkvQD/TM6B 		Tanimoto et al., 2000 N/A
D. melanogaster; w∗;
dally.mCh 		Norman et al., 2016 N/A
D. melanogaster;
w∗, BamΔ27::TIPF 		Michel et al., 2011 N/A
D. melanogaster;
dppHA/TM2 		Shimmi et al., 2005 N/A
D. melanogaster;
dppmCh 		Fereres et al., 2018 N/A
D. melanogaster;
tkvmCh 		gift from T. Kornberg N/A
D. melanogaster; w∗;
GAL4::VP16-nos 		Bloomington Cat# 4937; RRID: BDSC_4937
D. melanogaster;
dpp[hr92] cn[1] bw[1]/SM6a 		Bloomington Cat# 2069; RRID: BDSC_2069
D. melanogaster;
tkv[7] cn[1] bw[1] sp[1]/CyO 		Bloomington Cat# 106937; RRID: DGGR_106937
D. melanogaster;
w[∗]; Mad[1-2] P{ry[+t7.2]=neoFRT}40A/CyO 		Bloomington Cat# 7323; RRID: BDSC_7323
D. melanogaster;
ru[1] h[1] P{ry[+t7.2]=neoFRT}82B sr[1] e[s] Med[13]/TM3, Sb[1] 		Bloomington Cat# 7340; RRID: BDSC_7340
D. melanogaster;
w[1118]; PBac{y[+mDint2] w[+mC]=Dad-GFP.FLAG}VK00037 		Bloomington Cat# 42669; RRID: BDSC_42669
D. melanogaster;
w[∗]; P{w[+mC]=UAS-dpp.GFP.T}3/TM3, Sb[1] 		Bloomington Cat# 53716; RRID: BDSC_53716
D. melanogaster;
w[∗] P{w[+mC]=UAS-dally.J}SJ1 		Bloomington Cat# 5397; RRID: BDSC_5397
D. melanogaster;
w[∗]; P{w[+mC]=UASp-GFP.Act42A}5-5 		Bloomington Cat# 9252; RRID: BDSC_9252
D. melanogaster;
M{w[+mC]=UASp-LifeAct.mGFP6}ZH-2A, w[∗] 		Bloomington Cat# 58717; RRID: BDSC_58717
D. melanogaster;
P{w[+mC]=UASp-Act5C.mRFP}13, w[∗] 		Bloomington Cat# 24777; RRID: BDSC_24777
D. melanogaster;
w[1118]; P{w[+mC]=GAL4::VP16-nos.UTR}CG6325[MVD1], P{w[+mC]=UASp-GFPS65C-alphaTub84B}3 		Bloomington Cat# 7253; RRID: BDSC_7253
D. melanogaster;
w[∗]; sna[Sco]/CyO; P{w[+mC]=UASp-F-Tractin.tdTomato}10C/TM2 		Bloomington Cat# 58988; RRID: BDSC_58988
D. melanogaster; UASp-bamshRNA
y1 v1; P{TRiP.HMS00029}attP2 		Bloomington Cat# 33631; RRID: BDSC_33631
D. melanogaster; UASp-futschshRNA
y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMS02000}attP40 		Bloomington Cat# 40834; RRID: BDSC_40834
D. melanogaster; UASp-RfxshRNA
y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMJ23335}attP40 		Bloomington Cat# 61847; RRID: BDSC_61847
D. melanogaster; UASp-klp10AshRNA
y[1] sc[∗] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMS00920}attP2 		Bloomington Cat# 33963; RRID: BDSC_33963
D. melanogaster; UASp-diashRNA
y[1] sc[∗] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.GL00408}attP40/CyO 		Bloomington Cat# 35479; RRID: BDSC_35479
D. melanogaster; UASp-SCARshRNA
y[1] sc[∗] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01536}attP40 		Bloomington Cat# 36121; RRID: BDSC_36121
D. melanogaster; UASp-chershRNA
y[1] sc[∗] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMS01501}attP2/TM3, Sb[1] 		Bloomington Cat# 35755; RRID: BDSC_35755
D. melanogaster; UASp-nufshRNA
y[1] sc[∗] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMS02713}attP2 		Bloomington Cat# 43999; RRID: BDSC_43999
D. melanogaster; UASp-staishRNA
y[1] sc[∗] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.GL01099}attP2 		Bloomington Cat# 36902; RRID: BDSC_36902
D. melanogaster; UASp-Oseg6shRNA
y[1] sc[∗] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.GLC01452}attP2 		Bloomington Cat# 43263; RRID: BDSC_43263
D. melanogaster; UASp-IFT52shRNA
y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMJ22356}attP40 		Bloomington Cat# 58273; RRID: BDSC_58273
D. melanogaster; UASp-dallyshRNA
y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JF03175}attP2 		Bloomington Cat# 28747; RRID: BDSC_28747
Software and Algorithms
Fiji Schindelin et al., 2012 RRID: SCR 002285
GraphPad Prism 7 GraphPad Software RRID: SCR 002798
Deposited Data
RNA-seq data ArrayExpress E-MTAB-7063
Other
Leica TCS SP8 AOBS inverted microscope Leica N/A