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. 2019 Aug 1;35(8):710–717. doi: 10.1089/aid.2019.0039

FIG. 2.

FIG. 2.

Chemical inhibition of MDKs does not inhibit HIV transcription. (A) HeLa-CD4 cells were co-transfected with LTR-Luc and Tat for 48 h. CA was added at the indicated concentrations. Luciferase activity was measured 48 h post transfection, and is presented as a fold change in luciferase activity over untreated control cells (set to 1). (B) NH1 cells, stably expressing LTR-Luc, were transfected with Tat ± CA for 48 h. Luciferase activity was measured 48 h post transfection. (C) HeLa-CD4 cells were co-transfected with LTR-Luc and Tat for 48 h. SnxA was added at indicated concentrations. Luciferase activity was measured 48 h post transfection. (D) Whole-cell lysates of SnxA-treated HeLa-CD4 cells were separated on 15% SDS PAGE. The membrane was probed with anti-myc and anti-β-actin antibodies. Densitometry was calculated and normalized to β-actin. Results are representative of two independent experiments. Triplicate stimulations were performed for all experiments. Error bars represent standard errors of the mean. CA, Cortistatin A; LTR-Luc, long-terminal repeat luciferase; SnxA, Senexin A.