FIG. 3.

Chemical inhibition of MDKs decreases STAT1 phosphorylation. HeLa-CD4 cells were pretreated with (A) 10 nM CA for 1 h or (B) indicated concentrations of SnxA, then stimulated with IFNγ (10 ng/mL) for 1 h. Whole-cell lysates were run on 10% SDS-PAGE and blotted with anti-phospho-STAT1 and anti-STAT1 antibodies with β-actin serving as loading control. Representative blots of triplicate stimulations are presented. IFNγ, interferon-gamma.