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. 2019 Aug 1;35(8):710–717. doi: 10.1089/aid.2019.0039

FIG. 4.

FIG. 4.

Chemical inhibition of MDKs does not inhibit HIV reactivation. (A) J-Lat 8.4 cells were stimulated with PMA (12 nM) ± CA (200 nM) for 24 h. GFP expression of viable cells was measured by flow cytometry. (B) Percent viability was estimated using the percentage of live lymphocytes in 10,000 total cells analyzed. Untreated control cell viability was set at 100% viability. (C) 2D10 cells were stimulated with PMA (12 nM) ± SnxA (5 nM) for 24 h. GFP expression of viable cells was measured by flow cytometry. (D) Percent viability was estimated using the percentage of live lymphocytes in 10,000 total cells analyzed. Untreated control cell viability was set at 100% viability. Triplicate stimulations were performed. Error bars represent standard errors of the mean. GFP, green fluorescent protein; PMA, phorbol myristate acetate.