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. 2019 Aug 2;10:526. doi: 10.3389/fendo.2019.00526

Figure 6.

Figure 6

Cycloheximide (CHX) chase study. (A) DUOX1 protein expression was examined at different time periods in cells transfected with WT or p.R1307Q expression vectors. (B) DUOX1 expression was quantified densitometrically as the ratio of DUOX1/β-actin. (C) Degradation percentage of DUOX1 protein in cells transfected with WT or p.R1307Q expression vectors. Each column represents the mean ± SD of three independent experiments performed in triplicate.