TABLE 1.
Strains or plasmids | Relevant characteristics | Sources |
Strains | ||
E. coli W1688a | L-threonine producing strain | Prof. Sheng Yang |
E. coli W1688-fimH* | E. coli W1688 harboring plasmid pET28a-fimH | This study |
E. coli W1688-ΔfimH | E. coli W1688 with the deletion of fimH | This study |
E. coli W1688-pKD46 | E. coli W1688 harboring plasmid pKD46 | This study |
Plasmids | ||
pET28a | Kan resistance | Stored in our lab |
pET28a-fimH | pET28a containing fimH | This study |
pKD46 | Amp resistance | Stored in our lab |
pKD4 | Kan resistance | Stored in our lab |
aA gift from Prof. Sheng Yang (Institute of plant physiology and ecology, CAS, Shanghai, China).