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. 2019 Aug 2;10:1701. doi: 10.3389/fmicb.2019.01701

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Copyright © 2019 Galdino, Viganor, de Castro, da Cunha, Mello, Mattos, Pereira, Hunt, O’Shaughnessy, Howe, Devereux, McCann, Ramalho, Branquinha and Santos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of phendione-based compounds on P. aeruginosa lasB-induced cellular damage to lung epithelial cells (A549 line). Purified lasB (A) and pseudomonal conditioned supernatant (B) were previously incubated with phendione-based compounds at 25 μM for 1 h. Then, the lung epithelial cells were incubated for 48 h in the presence of P. aeruginosa supernatant (100 μg protein) or purified lasB (10 ng protein). After 48 h of incubation, A549 viability was analyzed using the MTT assay (A,B). In parallel, the integrity of the A549 monolayer and cell morphology were observed by light microscopy (C). The asterisks (*) denote significant difference between non-treated and treated cells (P < 0.05, ANOVA – Dunnett’s multiple comparison test), setting the untreated cells as a comparative parameter. < 0.05 – One-way ANOVA (Dunnett’s multiple comparison test), setting the supernatant-treated cells as a comparative parameter.

Inline graphic — Effects of phendione-based compounds on P. aeruginosa lasB-induced cellular damage to lung epithelial cells (A549 line). Purified lasB (A) and pseudomonal conditioned supernatant (B) were previously incubated with phendione-based compounds at 25 μM for 1 h. Then, the lung epithelial cells were incubated for 48 h in the presence of P. aeruginosa supernatant (100 μg protein) or purified lasB (10 ng protein). After 48 h of incubation, A549 viability was analyzed using the MTT assay (A,B). In parallel, the integrity of the A549 monolayer and cell morphology were observed by light microscopy (C). The asterisks (*) denote significant difference between non-treated and treated cells (P < 0.05, ANOVA – Dunnett’s multiple comparison test), setting the untreated cells as a comparative parameter. < 0.05 – One-way ANOVA (Dunnett’s multiple comparison test), setting the supernatant-treated cells as a comparative parameter.