Figure 2.

AZD1775 diminishes the growth of ESCC cells in vitro. (A) KYSE150 and EC109 cells were incubated with various concentrations of AZD1775 for 72 h. Cell viability was determined with MTT assay. Data from five independent experiments were shown. (B, C) KYSE150 and EC109 cells were exposed to increasing concentrations of AZD1775 for 48 h. The cells were then washed and subjected to focus colony formation (B) and soft agar assay (C) and incubated for 14 days, survival colonies were then counted. Data are expressed as mean ± SD, from six independent experiments.