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Examining effects over time for mice placed in an open field (anxiety-assay) and followed for the (a) number of entries into the center, (b) time spent in the center, and (c) distance moved. As indicated, C48/80 did not alter entries as compared to saline (e.g., did not increase anxiety) and sumatriptan resulted in a significant suppression of entries. Two-way ANOVA, post hoc Dunnett’s test: **P < 0.01; ***P<0.001 and ****P < 0.0001 compared to saline-treated group, N = 6.
