Table 1.

Crystal structures of allergen-antibody complexes

Antigen	Allergen expression system	Antibody	Antibody expression system	Co-purified by SEC	Crystallization Conditions	PDB code
Api m 2	Insect cells (High Five)d	Fab; IgG1	Mus musculus hybridoma cells	Yes	0.1M CHES pH 9.5, 0.2M NaCl, 10% PEG 8000	2j88 [12]
Bet v 1	E. coli	Fab′; IgG1	Mus musculus hybridoma cells	Yes	0.1M sodium citrate pH 4.0, 12% PEG 6000, 0.01% sodium azide	1FSK [14]
Bla g 2	Pichia pastoris	Fab′, 7C11, IgG1	Mus musculus hybridoma cells	Yes	0.1M sodium citrate pH 5.6, 16% PEG 10,000	2NR6 [18]
Bla g 2	Pichia pastoris	Fab′, 4C3, IgG1	Mus musculus hybridoma cells	Yes	Buffer unknown, 20% PEG 8000, 8% ethylene glycol, 0.2mM CdCl, 5mM DTT	3LIZ [22]
Bos d 5	E. coli	Fab	E. coli RV308	No	0.1M BTP pH 5.5, 14% PEG 3350	2R56 [13]
Der f 1	D. farinae mite culture	Fab; IgG1	Mus musculus hybridoma cells	Yes	0.1M HEPES pH 7.0,18% PEG 12,000	3RVV [17]
Der p 1	D. pteronyssinus mite culture	Fab; IgG1	Mus musculus hybridoma cells	Yes	1)0.1M sodium cacodylate pH 6.0, 15% PEG 4000 2)0.1M HEPES pH 7.0, 10% PEG4000, 0.05M GlyGlyGly	1)3RVW 2)3RVX [17]
Der p 1	D. pteronyssinus mite culture	Fab; IgG1	Mus musculus hybridoma cells	yes	0.1M MES pH 7.0, 10% PEG 6000, 5% MBP	4PP1 [16]
Der p 1	D. pteronyssinus mite culture	Fab; IgG1	Mus musculus hybridoma cells	Yes	0.1M sodium citrate pH 5.5, 15% PEG 6000	4PP2 [16]
Fel d 1	CHO	Fab; IgG4f	CHOc	Yes	0.1M sodium cacodylate pH 6.5, 0.2M calcium acetate, 40% PEG 300	5VYF [25]
Gal d 4 (lysozyme)	unknown	VH domain; Phage displayed	E. coli BL21 Goldb	Yes	1) 0.1M sodium citrate pH 5.4, 28% PEG 1500 2) 0.3M sodium citrate pH 5.5, 16% PEG 3350	1)4PGJ 2)4U3X [26]
Phl p 2	E. coli BL21e	Fab; IgE variable, IgG constant	CHO-K1	Yes	0.2M NaFormate, 20% PEG 3350	2VXQ [15]
Phl p 7	Ecoli BL21 star DE3a	Fab; IgG1	FreeStyle 293Fa	unknown	18 C, 0.1 M MES pH 6.5, 15% PEG 6000, 10% MPD	5OTJ [27]

^a)Life Technologies

^b)Stratagene

^c)Regeneron

^d)ThermoFisher

^e)Purchased from BIOMAY

^f)S228P, to promote disulfide bond stabilization