Fig. 5.

Knockdown of Egr1 reduced the levels of OPN secreted into the culture medium and decreased the migratory capability. a, Levels of OPN in the supernatants of Egr1 knockdown and control A549-Oct4 cells were quantified by ELISA. b, Migratory capabilities of Egr1 knockdown or control A549-Oct4 cells via lentivirus-mediated delivery of shRNA for 48 h. Cells that migrated through the membrane to the lower surface in the Boyden chamber were stained with Giemsa solution and visualized by light microscopy and photographed (left panel), and three fields in each membrane were counted (right panel). Data are mean ± SEM. *, p < 0.05; **, p < 0.01