Fig. 2.

Schematic overview of MVA-BAC technique for generation of recMVA. a In E. coli GS1783, following induction at 42 °C, the gene of interest together with I-SceI-Kan fragment is inserted into the DelVI site of the MVA-BAC by homologous recombination. Co-integrated Kan cassette (aphAI) is removed by induction of I-SceI production by arabinose followed by second Red recombination b In DF-1 cells, recMVAs are rescued in present of a helper virus and the BAC backbone including GFP cassette is removed spontaneously upon passaging