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. 2019 Aug 9;16:100. doi: 10.1186/s12985-019-1212-y

Table 1.

List of primers used in this study

Primer Sequence 5′ → 3´ Description
Kan control

F: CGTACTCCTGATGATGCATG

R: ATTCGTGATTGCGCCTGAGC

 For control PCR/sequencing after first recombination into MVA-BAC
MVA DelIII

F: GATGAGTGTAGATGCTGTTATTTTG

R: GCAGCTAAAAGAATAATGGAATTG

 To check the presence of WT and gene insertion after plaque picking
MVA DelVI

F: CTCCGCATCTAGTTGATATTCCAACCTCTT

R: CCTGGACATTTAGTTTGAGTGTTCCTGAAT

 For first homologous recombination into the MVA-BAC by En-passant
pEPH5-GII4

F: CATAAATAAGGTTGACTCTAGAGCCACCATGAAGATGGCCTC

R: ACGTAGAGCTCTTAAGGAATTCTTATACGGCTCGTCTTCTACCT

 For cloning of NoV GII.4 into pEP-MVAdVI-PH5 plasmid
pIIIH5-GII.4

F: TAAGGTTGACTCTAGAGCTAGCGCCACCATGAAGATGGCCTC

R: CGGCCGCGTTTAAACCTCGAGTTATACGGCTCGTCTTCTACCT

 For cloning of NoV GII.4 into the pIIIH5 shuttle vector
RFV

F: AAAGATGCGTACATTGGACCC

R: GTTCGAGACTAGAAAAGCGCC

 To check the presence of helper virus RFV in the transfect cells with recMVA-BAC