Fig. 3.

Butyrate treatment ameliorates LPC-induced demyelination in vitro. a Schematic showing the experimental protocol for demyelination analysis. b MBP and NF200 immunocytochemical staining of organotypic cerebellar slice cultures. Scale bar, 100 μm. c Myelination index (MI) of organotypic cerebellar slice cultures (n = 12 slices per group, pooled from four independent experiments). The box plot indicates the first and third quartiles and the middle line indicates the median. Whiskers indicate the minimum and maximum. *p < 0.05, ***p < 0.001