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. 2019 Aug 9;16:164. doi: 10.1186/s12974-019-1553-x

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© The Author(s). 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Expression analysis reveals comparatively higher TNFα compared to IL-1β receptor expression in differentiated astrocytes. a qRT-PCR confirms higher mRNA levels for canonical TNFα receptor gene TNFR1 (TNFRSF1A) compared to canonical IL-1β receptor IL1R1 in differentiated astrocytes. b Western blot confirms higher TNFR1 compared to IL1R1 protein expression in differentiated astrocytes (AST). Peripheral blood mononuclear cells (PBMC), and MOLT-4 cell lysates were included as positive TNFR1 and IL1R1 controls respectively