Fig. 5.

Modulated histone code in BML-278-treated immature GV oocytes. a Representative images of H3K9me3 and H3K4me2 subjected to the quantification of integrated density parameter via ImageJ software. b, c Quantification of integrated density of H3K9me3 and H3K4me2 localised and analysed in germinal vesicle (GV) of oocytes treated with BML-278 activator, respectively. The integrated density of BML-278-treated oocytes is normalised to the mean of the signal in control oocytes and expressed as a median ± quantiles from five independent experiments (N ≥ 30 oocytes per group Differences between individual group pairs were assessed post-hoc using multiple comparisons of mean ranks [30], including a Bonferroni adjustment for multiple testing; *, ** and *** denote significance at P ≤ 0.05, 0.01 and 0.005, respectively