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. 2019 Aug 9;19:59. doi: 10.1186/s12896-019-0553-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The purified protein was analyzed by 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Lane M—protein marker; lanes 1, 3, 5, 7—wild-type Ph-HepI and S169D, A259D and S169D/A259D mutants are produced by inducing the recombinant bacteria; lanes 2, 4, 6, 8—purified wild-type Ph-HepI and S169D, A259D and S169D/A259D mutants. The band of Ph-HepI protein (53 kDa) is indicated by an arrow