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. 2019 Aug 9;38:348. doi: 10.1186/s13046-019-1334-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — TET1-dependent demethylation induces transcriptional activation of SFRP2. a QRT-PCR of mRNA levels of DKKs and SFRPs in BX-TET1-OE and BX-TET1-NC cells. b Efficiency of si-SFRP2 was detected by qRT-PCR. c Transwell assays revealed that SFRP2 knockdown diminished TET1 inhibition of cell migration and invasion, **P < 0.01. d Western blot analysis of total β-catenin distribution after SFRP2 knockdown in BX-TET1-OE and BX-TET1-NC cells. e ChIP analysis of TET1 binding to the SFRP2 promoter. f 5-hmC and 5-mC content analysis in the SFRP2 promoter; MspI digestion indicated the 5-hmC level, HpaII digestion indicated 5-hmC and 5-mC levels, and the control indicated the total amount of 5-C, **P < 0.01. g BSP indicated the changes in methylation levels in the SFRP2 promoter