Fig. 2.

Apical infection of ZIKV is more efficient in Caco-2 cells. a Caco-2 cells infected at 3 pfu/ cell were assessed for ZIKV RNA expression at 6, 24, and 48 hpi, using SYBR-green qPCR assay and normalized to GAPDH expression. Results are expressed in mean ± SD calculated from three independent experiments. Data was analyzed using one way ANOVA *p < 0.05. b Caco-2 cells infected at 3 pfu/ cell were assessed for ZIKV-M protein expression at 48 hpi by Western Blot analysis. GAPDH was used as a loading reference