Fig. 3.

Strand-displacement activity of polymerase I large fragment from Psychrobacillus sp. at various temperatures. The strand-displacement activity assays have been performed in 50 mM BIS-Tris propane pH 8.5, 100 mM NaCl, 5 mM MgCl₂, 1 mM DTT, 0.2 mg/ml BSA and 2% glycerol. The increase in TAMRA fluorescence, i.e. enzyme activity, has been measured as relative fluorescence units (RFUs). For the reactions 100 ng protein were used. “Negative” represents the sample where no enzyme has been added and thus the negative control, performed at 25 °C