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. Author manuscript; available in PMC: 2019 Aug 9.
Published in final edited form as: J Vis Exp. 2018 Nov 1;(141):10.3791/58162. doi: 10.3791/58162

Figure 2. Constituent cell types are retained in isolated glomeruli.

Figure 2.

Confocal immunofluorescence microscopy was performed for nephrin (red, podocytes) and cell-specific markers to identify podocytes, mesangial cells, and the endothelium. (A) Costain for nephrin and WT1 (green, podocytes). (B) Costain for nephrin and PDGFR-β (green, mesangial cells, arrow). (C) Costain for nephrin and CD31 (green, endothelial cells, vessels in cross section marked by arrowheads). Scale bar = 25 μm on all panels.