Figure 4.
Susceptibility of RBM1 to various stressors as determined by disk diffusion assay. Plates with overlaid cultures were prepared as described in the text. Filter paper disks (6 mm) containing indicated stressors were placed on the agar plates. The plates were then incubated under microaerophilic conditions at 37°C for 16 h and inhibitory-zone diameters (indicated by arrows) were measured and compared. The stressors used are acriflavin (AF; 10 mg/ml), diquat dibromide (DQ; 500 mg/ml), ethidium bromide (EB; 10 mg/ml), hydrogen peroxide (H2O2; 10%), menadione (MN; 1%) methyl viologen (MV; 1.0 M), or pyronin B (PB; 0.5%). Assays were repeated twice and representative plates are shown.