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. Author manuscript; available in PMC: 2019 Aug 9.
Published in final edited form as: Cancer Immunol Res. 2018 Mar 27;6(5):605–616. doi: 10.1158/2326-6066.CIR-17-0314

Figure 3.

Figure 3.

OAd-BiTE-mediated oncolysis enhances the activation and proliferation of CART-cell preparations. FR-α+EGFR+ tumor cells were infected with OAd, OAd-BiTE, or left uninfected (mock), and after 24 hours, CART or UTD cells were added (E:T = 2). A and B, T cells were collected 48 hours after T-cell coculture and stained with antibodies against CD45, CD4, CD8, CAR, and CD25. B, CAR and CD25 expression on CART-cell preparations after coculture with tumor cells. C, Supernatants were obtained 48 hours after coculture, and IFNγ production was analyzed by ELISA. D, T-cell proliferation following coculture with tumor cells. The mean ± SD is shown in C and D. Representative of one of three independent experiments.