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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2019 Jun 8;1865(9):2428–2440. doi: 10.1016/j.bbadis.2019.06.006

Figure 3 – MiR-455-3p reduces APP and amyloid proteins.

Figure 3 –

(A) Representative immunostaining images of neuroblastoma cells transfected with the miR-control vector, the miR-455-3p vector, the mutant APP cDNA, the mutant APP and miR-455-3p co-transfected. Cells were stain with the APP (6E10) antibody producing red fluorescence (20× magnification). Green fluorescence showed the miR-control and miR-455-3p vector expression and the nucleus of the cell stained with DAPI (blue). Fluorescence intensity (red) of the mutant APP was reduce in mutant APP and miR-455-3p co-transfected cells. (B) Quantitative measurement of fluorescence intensity of the APP (6E10) protein in mutant APP, miR-455-3p, and miR-control vector transfected cells. (C) ELISA analysis for amyloid (1-40) and (1-42) levels in neuroblastoma cells. Quantitative detection of the (i) human amyloid-β(1-40) and (ii) amyloid-β(1-42) peptide level in mutant APP cDNA and miR-455-3p vector transfected cells. (*P<0.05)