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. 2019 Aug 2;10:1774. doi: 10.3389/fmicb.2019.01774

Figure 4.

Figure 4

Rough LPS accumulates more rapidly in mouse liver than does smooth LPS. Wild-type C57BL/6 mice were intravenously injected with 200 μl of saline containing either smooth (closed diamonds) or rough (open squares) LPS (20 μmol/L each). Mice were killed by heart puncture 3 or 24 h after injection (n = 4 for each group and each time point) and liver (A), spleen (B), and lung (C) were collected, immediately frozen in liquid nitrogen and stored at −80°C before further analysis. After homogenization, tissue levels of smooth and rough LPS were determined by direct quantitation of 3-hydroxymyristate (3HM) as described in “Experimental procedures.” Data are shown after subtraction of values obtained from mice injected with 200 μl of pyrogen-free saline and are mean ± SE. *Significantly different from smooth LPS, p < 0.05. See “Experimental Procedures” for additional experimental details.