Table 1.

p-Tvax epitopes.

N.	Antigen	Start position	Peptide	Length
1	KIF20A	860	Ac-SSTDSSPYARILRSRHSPLLK-amide	21
2	KIF2C	356	GDLSGKSQNASKGIYAMASRDVFLLKN-amide	27
3	MMP9	594	RVFFFSGRQMWVYTGKTVLGPRSLDKLGL-amide	29
4	MNDA	298	Ac-NETSSVLEAAPKQMIEVPNCITRN -amide	24
5	OLFML2B	50	DNQENVLSQLLGDYDKVKAVSEGSD-amide	25
6	TROAP	156	Ac-KGGTTQRGQSARSSAYLAPRIPTH-amide	24
7	ULBP1	56	Ac-LNRQPLFVYKDKKCHAIGAHRNSMNATKI-amide	29

Amino acid sequences of each antigen were screened with EpiMatrix to identify epitopes with potential immune stimulatory properties in BALB/c mice. Putative regulatory T cells epitopes (Tregitopes), which promote suppression of vaccine-induced T cell responses, and autoimmune epitopes (autoepitopes) that have potential of inducing autoimmune reactions were selected against using JanusMatrix algorithm. For each antigen, a peptide that contained both MHC-I and -II epitopes was synthetized as listed and used for vaccination, or for detection of antigen-specific immune responses.