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. Author manuscript; available in PMC: 2019 Aug 9.
Published in final edited form as: Prog Retin Eye Res. 2018 Oct 25;69:137–158. doi: 10.1016/j.preteyeres.2018.10.004

Fig. 2.

Fig. 2.

(A) ELOVL4 immunolabeling is detected in mouse retina, using affinity-purified ELOVL4 antibodies (green). Nuclei were stained with 4′,6-diamidino-2-phenylindole (blue). Images were captured by using an Olympus FluoView Confocal Microscope with 60X objective lens. IS, inner segments of rod and cone photoreceptors; ONL, outer nuclear layer. (B) Rat cardiomyocytes expressing ELOVL4 (red) or GFP (green) and non-transduced cells (blue) were cultured without precursors for 72 h. All cells, irrespective of ELOVL4 expression, synthesized C22-C26 PUFA. ELOVL4 expression in the absence of precursors resulted in elongation of endogenous precursor to C28-C38 VLC-PUFA. (C) Cardiomyocytes in B above, cultured with 20:5n3 synthesized C24-C26 in all treatment groups. Significant biosynthesis of C28-C38 n3 VLC-PUFA occurred in Elovl4-transduced cells (red), but not in GFP (green) and non-transduced cells (blue), with accumulation of 34:5n3 and 36:5n3. Note that each chromatogram was normalized to endogenous 20:1, which did not change among the sample groups. B & C are adapted reproductions from: Agbaga et al. (2008). Proceedings of the National Academy of Sciences, 105 (35) 12843–12848; DOI: 10.1073/pnas.0802607105.© 2008 by The National Academy of Sciences of the USA.