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. Author manuscript; available in PMC: 2019 Aug 9.
Published in final edited form as: Parasitol Int. 2018 Oct 17;68(1):87–91. doi: 10.1016/j.parint.2018.10.006

Fig. 2.

Fig. 2.

(A) Anti-PfRON12 rabbit antibodies have invasion-inhibitory activity in vitro. The ability of the anti-PfRON12 antibodies to inhibit the parasite invasion of erythrocytes was tested in a one-cycle GIA. Anti-EBA175 region 3–5 (EBA175) and anti-GST (GST) antibodies were used as positive and negative controls, respectively. The error bars represent the standard errors of the means of three independent experiments performed in triplicate. The Kruskal-Wallis test was performed, followed by Dunn’s multiple-comparison test, to compare percent inhibition by anti-GST and anti-PfRON12 antibodies in three different IgG concentrations. *; statistically significant. ns; not significant. (B) Human sera from malaria endemic areas in Mali and Thailand recognize PfRON12 in ELISA. The seropositivities were determined by the threshold of mean + 2 standard deviations (SD) in OD values against PfRON12 from Thai malaria-naïve sera (n = 10). The PfRON12 antigen was probed with samples from immune Malian adults (Mali), and from P. falciparum-infected asymptomatic and symptomatic Thai adults (Thailand (Asy) and Thailand (Sym), respectively). The number (n) of samples analyzed are shown.