Figure 3:

i2T2 can inject into the SCS and achieve large coverage despite anatomical variations (a) SCS coverage for injections performed with i2T2 is not influenced by variations in scleral thickness (Dotted red line indicates linear regression fit and shaded region shows 95% confidence interval. Pearson’s r test indicates non-significant correlation with P=0.76) (b) SCS coverage for injections performed with i2T2 is not influenced by variations in intraocular pressure. (Test: Ordinary one-way ANOVA) (c-e) MicroCT images of bovine, porcine, and rabbit eyes injected with contrast agent. Arrow indicates the location of injection. The experiments were repeated independently (n=3) with similar results. (f) For a given volume of injection (120 μl) ocular coverage increases for smaller eye size (Error bars indicate standard deviation). (g) SCS coverage can be improved by increasing the amount of fluid injected into the SCS (red dotted line indicates the line connecting mean values).