Table 1. Data-collection and refinement statistics.
Values in parentheses are for the highest resolution shell.
| Data collection | |
| Wavelength (Å) | 1.303 |
| Collected frames | 31000 |
| Diffraction hits | 1346 |
| Indexed frames | 1165 |
| Resolution range (Å) | 52.5–2.8 |
| Space group | I4122 |
| Unit-cell parameters (Å, °) | a = b = 53.6, c = 194.8, α = β = γ = 90 |
| Total No. of reflections | 164324 (2568) |
| No. of unique reflections | 3816 (368) |
| CC1/2 | 0.70 (0.245) |
| R split † (%) | 53.0 (142.1) |
| Completeness (%) | 99.9 (100.0) |
| Multiplicity | 3.0 (3.0) |
| Refinement | |
| Resolution range (Å) | 52.5–3.0 (3.30–3.00) |
| R work/R free (%) | 0.269 (0.2614)/0.304 (0.319) |
| No. of atoms | |
| RNA | 796 |
| Ligand | 43 |
| Ions/water | 2 |
| B factors (Å2) | |
| RNA | 45.7 |
| Ligand | 36.8 |
| Ions/water | 57.4 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.006 |
| Bond angles (°) | 1.141 |
†
R split is defined by CrystFEL (White et al., 2013 ▸) to be a figure of merit between two halves of a data set corrected for the loss in multiplicity.