O5(SL)6O5 hydrogel properties are pH and ionic strength independent. CD spectra of the positively charged MDP, K2(SL)6K2, neutral O5(SL)6O5, and negatively charged, E2(SL)6E2, at different pH conditions in water. K2 and E2 hydrogel formation is dependent on pH, whereas O5 remains a hydrogel at acidic (pH 3), neutral (pH 7), and basic pH (pH 11).