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. Author manuscript; available in PMC: 2020 Jul 31.
Published in final edited form as: J Am Chem Soc. 2019 Jul 5;141(30):11912–11922. doi: 10.1021/jacs.9b02963

Figure 5.

Figure 5.

Src’s interactome in different cellular states. (A) A schematic representation of co-clickable precipitation experiments performed in three distinct cellular contexts. Hela cells stably overexpressing Src V284C or Src WT were serum-starved, cultured in complete medium (DMEM) or plated on fibronectin. Prior to lysis, cells were incubated with 1-TCO or 2-TCO. Probe-bound Src complexes were enriched with Tz-beads and captured proteins were analyzed using LC-MS/MS. (B, C) Heat maps showing the enrichment score for each statistically significant interactor with 1-TCO- and 2-TCO-bound Src V284C under four different cellular conditions: (a) serum-starvation, (b) complete medium (DMEM), (c) growth factor stimulation, or (d) plating on fibronectin. The panel’s color scale indicates the mean enrichment score from three biological replicates. Cells colored in white are proteins that were not classified as significant interactors with the Src V284C-probe complex shown. (D) Venn diagrams representing common interactors in four cellular states for 1-TCO- (left) and 2-TCO-bound (right) Src.