Fig. 6.

T47D cells in 2D and 3D culture formats were incubated in the absence or presence of 1 mM DMOG for 1 h before culture in E2-deprived (Veh) or -supplemented (E2) medium (± DMOG) for 24 h. ELISA was used to quantify HIF-1α (A, B) and ERα (C, D) protein levels which were normalized to β-actin. ERα transcriptional activity was measured using the ONE-Glo luciferase activity assay (E, F). Data represent the average ± SEM from n ≥ 6 replicate cultures from at least two different cell passages.