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The interaction of neolymphostin with the PI3Ks is covalent. a) Incubation studies with PI3Kα and PI3Kγ performed in duplicate (first: grey; second: blue) b) Tandem MS/MS spectrum of a neolymphostin-modified PI3Kα peptide containing Lys802. Different b and y fragments and their location are indicated, with both the observed and expected masses of the precursor annotated.
