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. Author manuscript; available in PMC: 2020 Aug 8.
Published in final edited form as: Mol Cell. 2019 Jun 6;75(3):511–522.e4. doi: 10.1016/j.molcel.2019.05.014

Figure 1. 3’ uridylated isomiRs are associated with RISC.

Figure 1.

(A) After expressing a CMV-driven pri-miR-27a cassette in HEK293T cells, pre-miR-27a and mature miR-27a-3p were detected by Northern blotting with probe-miR-27a (Supplementary Table S1). (B) Small RNAs from HEK293T cells and HEK293T cells transfected with pri-miR-27a-expressing plasmids were extracted and subjected to deep sequencing. The average percentages of canonical miR-27a, its tailed isomiRs and trimmed isomiRs relative to the total miR-27a reads were plotted. Error bar indicates the standard deviation (n=3). (C) Nucleotide compositions of non-templated tail of miR-27a isomiRs in either HEK293T or HEK293T cells transfected with pri-miR-27a-expressing plasmids. (D) Small RNAs extracted from anti-AGO2 and anti-TNRC6C (GW182) immunoprecipitates were subjected to Northern blotting to detect miR-27a-3p. See also Figure S1.