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. Author manuscript; available in PMC: 2020 Aug 8.
Published in final edited form as: Cell. 2019 Jul 25;178(4):993–1003.e12. doi: 10.1016/j.cell.2019.06.031

Figure 3. Structures of NaVAb G94C/Q150C and Disulfide-crosslinked NaVAb V100C/Q150C in the Activated State.

Figure 3

(A) Overall structure of NaVAb/G94C/Q150C Δ28 activated state. The VS S0 to S3 segments are colored in orange and S4 in magenta. The S4-S5 linker is shown in blue and the pore module S5 to S6 in yellow. Ordered DMPC phospholipid molecules are highlighted in green for acyl chains and orange/red for phosphate head groups.

(B) Superposition of NaVAb/G94C/Q150C Δ28 structure with NaVAb/WT structure (PDB 4EKW). Cα backbone structures are shown with NaVAb/WT colored in gray with Cα r.m.s.d. of ~0.9 Å.

(C) Overall structure of NaVAb/V100C/Q150C Δ28 disulfide-crosslinked activated state. The structure is rendered as in (A). Difference in the C-terminal tail is due to crystallization pH condition (pH 4.8 vs. pH 5.6–6.0 for NaVAb/G94C/Q150C Δ28).

(D) Electron density map for V100C-Q150C disulfide bond. The FO−FC difference map is shown for V100C and Q150C side chains at 3.5σ (green) and 7σ (red) contour levels. Sulfur atoms on cysteine side chains are shown in gold.

See also Figure S4 and Table S1.