KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse Monoclonal Anti-FLAG M2 Antibody	Sigma-Aldrich	Cat# F1804
Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)	Jackson ImmonuResearch Laboratories	Cat# 115–035-003
Bacterial and Virus Strains
E. coli BL21(DE3)	NEB	Cat# C2527I
E. coli DH10Bac	ThermoFisher Scientific	Cat# 10361–012
Chemicals, Peptides, and Recombinant Proteins
Gramicidin	Sigma-Aldrich	Cat# G5002
Digitonin, High Purity	Millipore Sigma	Cat# 300410
1,2-Dimyristoyl-sn-Glycero-3-Phosphocholine (DMPC)	Anatrace	Cat# D514
Anti-FLAG M2 Affinity Gel	Millipore Sigma	Cat# A2220
FLAG peptide DYKDDDDK	Bio Basic	N/A
Deposited Data
Coordinates and Structure Factors of NaVAb G94C/Q150C ∆28	This study	PDB: 6P6X
Coordinates and Structure Factors of NaVAb V100C/Q150C ∆28	This study	PDB: 6P6Y
Coordinates of NaVAb KAV/G94C/Q150C	This study	PDB: 6P6W
Cryo-EM Map of NaVAb KAV/G94C/Q150C	This study	EMDB: EMD-20265
Experimental Models: Cell Lines
Spodoptera frugiperda (Sf9)	Life Technologies	B825–01
Trichoplusia ni (High Five)	Life Technologies	B855–02
Recombinant DNA
pET-21b FLAG-NaVAb	This study	N/A
pFastBacDual FLAG-NaVAb	Payandeh et al., 2012	N/A
pFastBacDual FLAG-NaVAb G94C/Q150C ∆28	This study	N/A
pFastBacDual FLAG-NaVAb V100C/Q150C ∆28	This study	N/A
pFastBacDual MBP-NaVAb KAV/G94C/Q150C	This study	N/A
pIZT GFP-P2A-NaVAb	This study	N/A
Software and Algorithms
HKL2000	HKL Research	http://www.hkl-xray.com
CCP4i 7.0.068	CCP4	http://www.ccp4.ac.uk
Coot 0.8.9.1	MRC	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot
Leginon 3.3	NRAMM	http://leginon.org
MotionCor2	UCSF	https://msg.ucsf.edu/software
Gctf	MRC	https://www.mrc-lmb.cam.ac.uk/kzhang
Relion 3.0 beta	MRC	https://www2.mrc-lmb.cam.ac.uk/relion
CisTEM 1.0.0-beta		https://cistem.org
MonoRes	Scipion	http://scipion.cnb.csic.es/m/myresmap
Chimera 1.11.2	UCSF	https://www.cgl.ucsf.edu/chimera
Phenix 1.14–3260		https://www.phenix-online.org
IGOR Pro 6.37	WaveMetrics	https://www.wavemetrics.com
Pymol 1.7.2	Schrödinger	https://pymol.org