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. Author manuscript; available in PMC: 2020 Aug 15.
Published in final edited form as: Arch Biochem Biophys. 2019 Jun 26;671:103–110. doi: 10.1016/j.abb.2019.06.006

Fig. 2. Non-ionic detergents inhibit HisACAT1/FLAG activity at and above their CMCs.

Fig. 2

The enzymatic inhibition of purified HisACAT1/FLAG (liposomal ACAT activity assay) by Triton X–100 (n = 3) and octyl glucoside (n = 3). Liposomal ACAT activity assay was performed in 100 mM KCl and 5 mM CHAPS. ACAT activity (%) was determined by normalization of each experimental trial to background [3H]cholesteryl oleate (0%) and activity at 100 mM KCl, 5 mM CHAPS (100%) of that trial. (B) The mean diameter (Z-average) of particles, as determined by dynamic light scattering, found in 100 mM KCl, 5 mM CHAPS, 50 mM Tris-HCl pH 7.8, 6.3 mM PC, 5.2 mM taurocholate, and 1 mM cholesterol with added concentrations of Triton X–100 (TX100) or octyl glucoside (OG).