Figure 4.

Cellular communication network factor 2 (CCN2) in flexor digitorum muscles. (A–C) Immunoexpression and enzyme‐linked immunosorbent assay (ELISA) detected levels in muscles and serum. (D) An untreated 3‐week high repetition high force (HRHF) muscle probed for both SMA and CCN2 immunoexpression. Only the arteriole shown in the insets shows co‐localization. (E) Location of CCN2 immunoexpression in muscle cross‐sections. (F) Anti‐human IgG detection of the FG‐3019 agent in muscles of HRHF + FG‐3019 rats; staining absent in untreated HRHF rats. a: p < 0.05 and aa: p < 0.01, compared with matched FRC group; b: p < 0.05 and bb: p < 0.01, compared with HRHF rats; cc: p < 0.01, compared with HRHF + hIgG rats. Scale bars = 50 μm. The 0‐week HRHF rat results from Supplementary Table 1 are indicated with dotted lines [Color figure can be viewed at wileyonlinelibrary.com]