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. Author manuscript; available in PMC: 2020 Aug 8.
Published in final edited form as: Mol Cell. 2019 Jun 5;75(3):442–456.e4. doi: 10.1016/j.molcel.2019.05.011

Figure 6. RTN3-mediated ER-phagy clears mutant prohormone aggregates.

Figure 6.

A. HEK 293T cells expressing C28F POMC-FLAG were co-transfected with the indicated siRNAs, and with either Sec61β-GFP or RTN3C-GFP. Soluble and insoluble fractions were generated as in 1C. The bottom graph represents insoluble C28F POMC-FLAG level normalized to the level of insoluble C28F POMC-FLAG in the scrambled control + Sec61β-GFP. Data represent the mean ± SD (n=3); p-values generated from student’s T-test. B. As in A, except cells were transfected with G57S Pro-AVP-FLAG. The bottom graph represents insoluble G57S Pro-AVP-FLAG level normalized to the level of insoluble G57S Pro-AVP-FLAG in the scrambled control + Sec61β-GFP (n=3).