Fig. 1. A small-molecule inhibitors library is used to identify potential agents against CCA.

Initial screening yielded 34 primary hit compounds that caused over a 50% viability reduction in all three CCA cell lines (HuCCT1, HuH28, and RBE). Second screening yielded 20 primary hit compounds that caused over a 50% viability reduction in all tested CCA cell lines. Subsequent triplicate, dose-dependent analysis was performed to measuring the IC₅₀ values of above 20 compounds. Heatmap shows the list of the most effective 12 compounds according to the unbiased small-molecule inhibitors screening