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. 2019 Aug 9;10(8):602. doi: 10.1038/s41419-019-1831-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a CDK7 mRNA expression profile based on tumor/normal fold change in different tumors in TCGA. b CDK7 mRNA expression is upregulated in CCA in TCGA. c–g CDK7 mRNA expression in CCA is higher compared with that in normal biliary tissues or non-tumor liver tissues in GSE26566 dataset, GSE107943 dataset, GSE32225 dataset, GSE76297 dataset and GSE32879 dataset. Data presented were log2 normalized. h Immunohistochemical staining for CDK7 performed on 88 human cholangiocarcinoma tissues and 29 adjacent non-tumor tissues. Representative images (magnification, × 200 and 400) were shown. i Semi-quantitative analysis of immunohistochemical staining shows that CDK7 protein expression is upregulated in CCA in the tissue microarray. Mann–Whitney test was performed to compare two groups. j Bar graph shows the percentage of CDK7 positive expression ratio in tumor and non-tumor tissues in the tissue microarray