Fig. 3.
Expression of VIM-2 and SPM-1 in non-frequent hosts induces envelope stress. a DegP-3xFLAG protein levels by western blot, using anti-FLAG antibodies, in whole cells from degP-3xFLAG E. coli cells carrying the empty vector or expressing each MBL, at 20 µM of IPTG. Short- DegP is showed in cells expressing blaVIM-2 or blaSPM-1. This form of DegP indicates that mature DegP was self-cleaved in vivo. GroEL was used as a load control. This panel is representative of two independent experiments. b Growth curves of the degP null (∆degP) and wild-type E. coli strains, carrying the empty vector (Vector) or expressing blaNDM-1, blaVIM-2, or blaSPM-1 in aerobic conditions in LB medium at 37 °C with 20 µM of IPTG. The growth was monitored as above. Data correspond to three independent experiments and are shown as the mean value. Error bars represent standard deviations (s.d). Source data are provided as a Source Data file