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. 2019 Aug 9;9:11580. doi: 10.1038/s41598-019-48043-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A–C) Combined ROC analysis between HCC and cirrhosis induced by NASH (A), ALC (B), and HCV (C), respectively (blue dash line, ROC curve of trifucosylation; green dash line, AFP; orange dash line, INR; red solid line, trifucosylated AGP and AFP combined; purple solid line, trifucosylated AGP and INR combined). (D) 2D scatter plot of the trifucosylation degree of serum AGP and the clinical AFP value in cirrhosis (blue) and HCC (red) patients with AFP < 20 ng/mL. Each spot represents an individual patient. HCC patients with negative AFP but high level of the trifucosylated AGP are marked with arrows.